Imaging Facility

Sharan Atwal
Public Health Research Institute
International Center for Public Health
New Jersey Medical School
Rutgers, The State University of New Jersey
225 Warren Street
Newark, New Jersey 07103, USA

Room W420J.2
Phone: (973) 854-3412


Welcome to the PHRI imaging facility! We provide access to high quality microscopy for users at Rutgers University and external users. As well as imaging, users can be trained to allow independent imaging and data processing. For your convenience, sample preparation and image acquisition can also be carried out by our staff to ease your workload! Before imaging, a consultation is always given to allow you to gain the best possible results.

Imaging Equipment

Leica LMD6

  • LMD6 laser micro dissection system with fully automated upright DM6 microscope
  • Scanning stage allows for specimen overview of merged image fields for easier specimen navigation
  • CC7000 monochrome camera
  • Objectives ranging from 1.25X to 63x
  • Contrast imaging techniques including Transmitted light Brightfield, Transmitted light DIC, Fluorescence

Leica SP8 confocal

  • DMi8 inverted microscope
  • Closed Loop Adaptive Focus Control (AFC) for long time-lapse imaging
  • Objectives: 10x/0.3, 20x/0.75, 40x/0.6 CORR, 40x/1.3 oil, 63x/1.4 oil
  • Interference Contrast
  • Motorized scanning stage
  • LAS X Navigator for sample exploration, tile scanning, and Assay Editor for multiwell and multi-chamber experiments
  • Laser lines: 405, 488, 552, 638 nm
  • 5 spectrally tunable (1nm) detector channels including Leica’s HyD Hybrid Detector
  • LAS X Lightning super-resolution module (down to 120nm resolution)

Nikon Eclipse 90i

  • Light microscopy
  • Motorised upright with XY stage
  • Light, DIC and epifluorescence modes
  • Dual camera ports
  • Hamamatsu Orca Flash 4.0 v2 CMOS monochrome camera
  • NIS Elements Digital Imaging software
  • Objectives 10x, 20x, 40x and 60x

Pre-imaging Consultation

This will be free and cover questions such as:

  • What is the goal of the imaging? What question do you want to answer, or what phenomenon do you want to illustrate with images?
  • Do you know which microscope you would like to use?
  • What is the sample?
  • How is the sample mounted?
  • For fluorescence imaging, what labels are you using?
  • Do you need transmitted-light (bright-field) imaging?
  • Is there anything else we should know about your sample or project?
  • Do you need to live imaging?
  • What microscopy experience do you already have?


The use of equipment and services are subject to a user fee.  Equipment time and services are available on an hourly-based schedule. Please contact Sharan Atwal for more details.

Fee per hour ($) Internal External
Consultation 0 0
Leica LMD6 10 20
Leica SP8 confocal 10 20
Nikon Eclipse 90i 10 20
Training by staff 10 20
Assistance by staff after training 20 40
Fee per sample* ($) Internal External
Sample preparation by staff 50 100
Sample imaged by staff 50 100
Sample preparation and microscopy by staff 100 200
Image analysis by staff 50 100
Basic reagents (glass slides, coverslips, mounting media etc) 0 0

*One sample is considered as <10 conditions.

In order to reserve a time slot in the imaging facility, please download Outlook.pdf and follow the instructions to add the Microscope Calendar to your Office Outlook App.

Additional Information

Important: Because projects carried out in the facility involves pathogens, we require adherence to regulations or approvals by standard operating procedures (SOP), institutional review boards (IRB) and institutional biosafety committees (IBC) for all experiments.  For additional questions regarding biosafety contact Jessica McCormick: office phone (973) 972-8424.

All users require basic training before using any microscope or equipment in our facility. Advanced training available for certain equipment.

Discussion of a project with PHRI staff prior to starting is advised in order to obtain useful and high-quality data, as well as to discuss potential biohazard concerns.