Imaging Facility
Sharan Atwal
Public Health Research Institute
International Center for Public Health
New Jersey Medical School
Rutgers, The State University of New Jersey
225 Warren Street
Newark, New Jersey 07103, USA
Room W420J.2
Phone: (973) 854-3412
e-mail: sa1399@njms.rutgers.edu
Overview
Welcome to the PHRI imaging facility! We provide access to high quality microscopy for users at Rutgers University and external users. As well as imaging, users can be trained to allow independent imaging and data processing. For your convenience, sample preparation and image acquisition can also be carried out by our staff to ease your workload! Before imaging, a consultation is always given to allow you to gain the best possible results.
Imaging Equipment
Leica LMD6
- LMD6 laser micro dissection system with fully automated upright DM6 microscope
- Scanning stage allows for specimen overview of merged image fields for easier specimen navigation
- CC7000 monochrome camera
- Objectives ranging from 1.25X to 63x
- Contrast imaging techniques including Transmitted light Brightfield, Transmitted light DIC, Fluorescence
Leica SP8 confocal
- DMi8 inverted microscope
- Closed Loop Adaptive Focus Control (AFC) for long time-lapse imaging
- Objectives: 10x/0.3, 20x/0.75, 40x/0.6 CORR, 40x/1.3 oil, 63x/1.4 oil
- Interference Contrast
- Motorized scanning stage
- LAS X Navigator for sample exploration, tile scanning, and Assay Editor for multiwell and multi-chamber experiments
- Laser lines: 405, 488, 552, 638 nm
- 5 spectrally tunable (1nm) detector channels including Leica’s HyD Hybrid Detector
- LAS X Lightning super-resolution module (down to 120nm resolution)
Nikon Eclipse 90i
- Light microscopy
- Motorised upright with XY stage
- Light, DIC and epifluorescence modes
- Dual camera ports
- Hamamatsu Orca Flash 4.0 v2 CMOS monochrome camera
- NIS Elements Digital Imaging software
- Objectives 10x, 20x, 40x and 60x
Pre-imaging Consultation
This will be free and cover questions such as:
- What is the goal of the imaging? What question do you want to answer, or what phenomenon do you want to illustrate with images?
- Do you know which microscope you would like to use?
- What is the sample?
- How is the sample mounted?
- For fluorescence imaging, what labels are you using?
- Do you need transmitted-light (bright-field) imaging?
- Is there anything else we should know about your sample or project?
- Do you need to live imaging?
- What microscopy experience do you already have?
Fees
The use of equipment and services are subject to a user fee. Equipment time and services are available on an hourly-based schedule. Please contact Sharan Atwal for more details.
| Fee per hour ($) | Internal | External |
|---|---|---|
| Consultation | 0 | 0 |
| Leica LMD6 | 10 | 20 |
| Leica SP8 confocal | 10 | 20 |
| Nikon Eclipse 90i | 10 | 20 |
| Training by staff | 10 | 20 |
| Assistance by staff after training | 20 | 40 |
| Fee per sample* ($) | Internal | External |
|---|---|---|
| Sample preparation by staff | 50 | 100 |
| Sample imaged by staff | 50 | 100 |
| Sample preparation and microscopy by staff | 100 | 200 |
| Image analysis by staff | 50 | 100 |
| Basic reagents (glass slides, coverslips, mounting media etc) | 0 | 0 |
*One sample is considered as <10 conditions.
In order to reserve a time slot in the imaging facility, please download Outlook.pdf and follow the instructions to add the Microscope Calendar to your Office Outlook App.
Additional Information
Important: Because projects carried out in the facility involves pathogens, we require adherence to regulations or approvals by standard operating procedures (SOP), institutional review boards (IRB) and institutional biosafety committees (IBC) for all experiments. For additional questions regarding biosafety contact Jessica McCormick: office phone (973) 972-8424.
All users require basic training before using any microscope or equipment in our facility. Advanced training available for certain equipment.
Discussion of a project with PHRI staff prior to starting is advised in order to obtain useful and high-quality data, as well as to discuss potential biohazard concerns.