Flow Cytometry & Mass Spectrometry Facility

Blas Peixoto
Public Health Research Institute
International Center for Public Health
New Jersey Medical School – Rutgers, The State University of New Jersey
225 Warren Street
Newark, New Jersey 07103, USA

Phone: (973) 854-3226
e-mail: peixotbp@njms.rutgers.edu

Flow Cytometry Services

The PHRI Flow Cytometry Core Facility is equipped with 2 bench top cell analyzers:

  1. BD FACS Celesta with High Throughput Sampler (HTS).  The system is equipped with 3 lasers (405 nm, 488nm, and 640 nm). The laser/filter configuration allow to detect and analyze up to 14 parameters including FSC and SSC. The HTS option supports a wide range of research applications and is compatible with 96-well U, V, and flat-bottom plates as well as 384-well microtiter plates.

BD FACS Celesta filter configuration

Laser Filter Example fluorophores
405 nm 450/40 BP BV421, V450, Pacific Blue, Hoechst 33342, DAPI
525/50 BP – 505 LP BV510, V500, AmCyan
610/20 BP – 595 LP BV605
670/30 BP – 655 LP BV650
780/60 BP – 750 LP BV786
488 nm 530/30 BP – 505 LP FITC, GFP, Alexa 488, BB515, CFSE
575/25 BP – 550 LP PE
610/20 BP – 600 LP PE-CF594, PI, RFP
695/40 BP – 670 LP PerCP, PerCP-Cy5, 7-AAD
780/60 BP – 750 LP PE-Cy7
640 nm 670/30 BP APC, Alexa647, Cy5
730/45 BP – 690 LP APC-R700, Alexa700
780/60 BP – 750 LP APC-Cy7
  1. BD FACSVia.  The flow cytometer is equipped with two lasers (488nm and 640 nm) and allows to detect up to 6 parameters including FSC and SSC. The BD FAVSVia loader allows to acquire samples from FACS tubes as well as 96-well plates.

BD FACSVia Filter configuration

Laser Filter Example fluorophores
488 nm FL1 533/30 BP FITC, GFP, Alexa 488, BB515, CFSE, JC-1, Flou-4AM, SYBR Green, YFP
FL2 585/40 BP PE, PI, JC-1, BD MitoStatus TMRE, OFP
FL3 670 LP PerCP, PerCP-Cy5.5, PE-Cy7, PE-Cy5, BD Horizon PE-CF594, RFP, mCherry, dsRed, 7-AAD, PE-Cy5
640 nm FL4 675/25 BP APC, Alexa647, Bd MitoStatus Red, APC-H7, APC-Cy7

Mass Spectrometry Services

The PHRI Mass Spectrometry Core Facility is equipped two mass spectrometers. The first platform includes an Agilent 1260 Infinity liquid chromatography system, with standard pump pressure and UV detector, coupled to an Applied Biosystems (ABI) MDS Sciex 4000 QTRAP tandem mass spectrometer. The combination of the triple quadrupole with Linear Ion Trap technology (LIT) offers superior sensitivity and performance. The second platform is a Shimadzu Prominence HPLC with autosampler coupled to an Applied Biosystems (ABI) MDS Sciex 4000. Both systems are powered by the Analyst and BioAnalyst software with built-in automation capabilities to simplify method development data acquisition and data processing with two bench top cell analyzers.


The use of equipment and services are subject to a user fee.  Equipment time and services are available on an hourly-based schedule. Please contact Blas Peixoto to book a time slot and/or an introduction to the equipment.

Additional Information:

Important: Because projects carried out in the facility involves pathogens, we require adherence to regulations or approvals by standard operating procedures (SOP), institutional review boards (IRB) and institutional biosafety committees (IBC) for all experiments.  For additional questions regarding biosafety contact Jessica McCormick: office phone (973) 972-8424.

All users require basic training before using any equipment in our facility. Advanced training available for certain equipment.

Discussion of a project with PHRI staff prior to starting is advised in order to obtain useful and high-quality data, as well as to discuss potential biohazard concerns.