Flow Cytometry & Mass Spectrometry Facility
Blas Peixoto
Public Health Research Institute
International Center for Public Health
New Jersey Medical School – Rutgers, The State University of New Jersey
225 Warren Street
Newark, New Jersey 07103, USA
Phone: (973) 854-3226
e-mail: peixotbp@njms.rutgers.edu
Flow Cytometry Services
The PHRI Flow Cytometry Core Facility is equipped with 2 bench top cell analyzers:
- BD FACS Celesta with High Throughput Sampler (HTS). The system is equipped with 3 lasers (405 nm, 488nm, and 640 nm). The laser/filter configuration allow to detect and analyze up to 14 parameters including FSC and SSC. The HTS option supports a wide range of research applications and is compatible with 96-well U, V, and flat-bottom plates as well as 384-well microtiter plates.
BD FACS Celesta filter configuration
| Laser | Filter | Example fluorophores |
| 405 nm | 450/40 BP | BV421, V450, Pacific Blue, Hoechst 33342, DAPI |
| 525/50 BP – 505 LP | BV510, V500, AmCyan | |
| 610/20 BP – 595 LP | BV605 | |
| 670/30 BP – 655 LP | BV650 | |
| 780/60 BP – 750 LP | BV786 | |
| 488 nm | 530/30 BP – 505 LP | FITC, GFP, Alexa 488, BB515, CFSE |
| 575/25 BP – 550 LP | PE | |
| 610/20 BP – 600 LP | PE-CF594, PI, RFP | |
| 695/40 BP – 670 LP | PerCP, PerCP-Cy5, 7-AAD | |
| 780/60 BP – 750 LP | PE-Cy7 | |
| 640 nm | 670/30 BP | APC, Alexa647, Cy5 |
| 730/45 BP – 690 LP | APC-R700, Alexa700 | |
| 780/60 BP – 750 LP | APC-Cy7 |
- BD FACSVia. The flow cytometer is equipped with two lasers (488nm and 640 nm) and allows to detect up to 6 parameters including FSC and SSC. The BD FAVSVia loader allows to acquire samples from FACS tubes as well as 96-well plates.
BD FACSVia Filter configuration
| Laser | Filter | Example fluorophores |
| 488 nm | FL1 533/30 BP | FITC, GFP, Alexa 488, BB515, CFSE, JC-1, Flou-4AM, SYBR Green, YFP |
| FL2 585/40 BP | PE, PI, JC-1, BD MitoStatus TMRE, OFP | |
| FL3 670 LP | PerCP, PerCP-Cy5.5, PE-Cy7, PE-Cy5, BD Horizon PE-CF594, RFP, mCherry, dsRed, 7-AAD, PE-Cy5 | |
| 640 nm | FL4 675/25 BP | APC, Alexa647, Bd MitoStatus Red, APC-H7, APC-Cy7 |
Mass Spectrometry Services
The PHRI Mass Spectrometry Core Facility is equipped two mass spectrometers. The first platform includes an Agilent 1260 Infinity liquid chromatography system, with standard pump pressure and UV detector, coupled to an Applied Biosystems (ABI) MDS Sciex 4000 QTRAP tandem mass spectrometer. The combination of the triple quadrupole with Linear Ion Trap technology (LIT) offers superior sensitivity and performance. The second platform is a Shimadzu Prominence HPLC with autosampler coupled to an Applied Biosystems (ABI) MDS Sciex 4000. Both systems are powered by the Analyst and BioAnalyst software with built-in automation capabilities to simplify method development data acquisition and data processing with two bench top cell analyzers.
Fees
The use of equipment and services are subject to a user fee. Equipment time and services are available on an hourly-based schedule. Please contact Blas Peixoto to book a time slot and/or an introduction to the equipment.
Additional Information:
Important: Because projects carried out in the facility involves pathogens, we require adherence to regulations or approvals by standard operating procedures (SOP), institutional review boards (IRB) and institutional biosafety committees (IBC) for all experiments. For additional questions regarding biosafety contact Jessica McCormick: office phone (973) 972-8424.
All users require basic training before using any equipment in our facility. Advanced training available for certain equipment.
Discussion of a project with PHRI staff prior to starting is advised in order to obtain useful and high-quality data, as well as to discuss potential biohazard concerns.