Maria Gennaro2018-11-05T18:59:20+00:00

Maria Laura Gennaro, M.D.

Interim Director of PHRI
Professor of Medicine
marila.gennaro@rutgers.edu   |  View CV
+1-973-854-3210
room W250 Q-W

NEWS:

Read our paper on disease-specific biogenesis of foam cells https://journals.plos.org/plospathogens/article?id=10.1371/journal.ppat.1007223

See how RNA flow cytometry identifies quantitative differences in T cell functional status between latent M. tuberculosis infection and active tuberculosis https://www.ncbi.nlm.nih.gov/pubmed/30283456

A clinical trial in South Africa to test statins as host-directed therapy for tuberculosis, in which we participate, has been funded.

Our FISH-Flow protocol can be found here.

Mycobacterium tuberculosis is an intracellular pathogen that infects the host macrophage. The most common infection outcome is a standoff in which both pathogen and macrophage undergo adaptive modifications, and survive. Maintaining this equilibrium between bacteria and innate immune cells requires a third player, the host acquired immune (T-cell-mediated) responses. When the T cell response is compromised, the host-pathogen equilibrium is broken, and disease ensues. To date, two billion people carry latent (asymptomatic) M. tuberculosis infection; of these, 10 million become sick every year with tuberculosis, and one and a half million die.

Our laboratory studies mechanisms of adaptation expressed by the bacterium and by the host macrophage during infection, with the goal of finding targets for therapeutic intervention. We also develop tools that may help identify latently infected individuals that are at high risk of developing active tuberculosis.

Macrophage lipid metabolism:

Foam cells are lipid-laden macrophages associated with chronic inflammatory states and tissue damage in many pathological (infectious and non-infectious) conditions. In tuberculosis, foam cells are central to establishing and maintaining chronic Mycobacterium tuberculosis infection. During active tuberculosis, the abundance of foam cells correlates with the extent of tissue damage, linking these cells to immunopathology. Our laboratory studies the biogenesis and key characteristics of foam cells during M. tuberculosis infection, and the interplay between metabolic and inflammatory stimuli leading to their formation.

Statins, which are inhibitors of the mevalonate pathway and de novo cholesterol synthesis, exhibit anti-mycobacterial activity in macrophages infected in vitro and increase the bactericidal efficacy of standard anti-tuberculosis chemotherapy in mice. We are currently studying the mechanisms underlying the observed anti-mycobacterial activity of statins and the interplay between metabolic and inflammatory effects of statin treatment on M. tuberculosis infection. Our work has led to an ongoing, NIH-funded clinical trial in South Africa to assess the effectiveness of statins as adjunctive, host-directed therapy against tuberculosis.

M. tuberculosis stress responses:

Our laboratory also investigates links between regulatory and metabolic adaptive responses of M. tuberculosis during infection. We have shown that expression of lung immunity in mice is associated with upregulation of a “dormancy” regulon, changes in expression of genes involved in central carbon metabolism and respiration, and mycobacterial cell wall remodeling. By collaborating with mathematical modelers and network analysts, we reconstructed the complete sigma factor network of tubercle bacilli and dissected regulatory links and dynamics between the envelope-stress-response network and regulation of central metabolism genes of M. tuberculosis. We also discovered that M. tuberculosis possesses the phage shock protein (Psp) system, a multi-protein system found across bacterial phyla that stabilizes the cell envelope during stress conditions. We study the correlations between Psp function, proton motive force preservation, ATP homeostasis, susceptibility to anti-tuberculosis antibiotics, and mycobacterial virulence. In addition, we are studying the phylogenetic spread, domain architecture, and genomic context of the elements of the Psp system in the bacterial kingdom with the goal of linking evolution of protein and operon structure to function.

FISH-Flow:

We have developed an RNA-flow cytometry assay (FISH-Flow) which allows to simultaneously measure mRNA and protein in single cells.  The method has advantages over in-situ fluorescence microscopy (FISH) and conventional (antibody-based) flow cytometry. Moreover, the nucleic acid probes we use (“Stellaris”) afford extremely low background signals, which makes our protocol more suitable for detection of rare events than RNA flow cytometry techniques utilizing other types of nuclei acid probes. We apply FISH-Flow to detection of antigen-specific T cell responses in tuberculosis.  The methodology can also be applied to other cell types, to immune responses in other pathological conditions, and to viral detection. In collaboration with BD Biosciences, we have developed a semi-automated FISH-Flow protocol that reduces labor and cell loss and increases assay reproducibility and its clinical applicability.

View all on Pubmed

Guerrini V, Prideaux B, Blanc L, Bruiners N, Arrigucci R, Singh S, Ho-Liang HP, Salamon H, Chen PY, Lakehal K, Subbian S, O’Brien P, Via LE, Barry CE, 3rd, Dartois V, Gennaro ML (2018) Storage lipid studies in tuberculosis reveal that foam cell biogenesis is disease-specific. PLoS Pathog 14: e1007223. PMI: 30161232

Arrigucci R, Lakehal K, Vir P, Handler D, Davidow AL, Herrera R, Estrada-Guzman JD, Bushkin Y, Tyagi S, Lardizabal AA, Gennaro ML (2018) Active Tuberculosis Is Characterized by Highly Differentiated Effector Memory Th1 Cells. Front Immunol 9: 2127. PMI: 30283456

Lakehal K, Levine D, Kerr KF, Vir P, Bruiners N, Lardizabal A, Gennaro ML, Pine R (2017) Tuberculosis State Is Associated with Expression of Toll-Like Receptor 2 in Sputum Macrophages. mSphere 2. PMI: 29104936

Broger T, Basu Roy R, Filomena A, Greef CH, Rimmele S, Havumaki J, Danks D, Schneiderhan-Marra N, Gray CM, Singh M, Rosenkrands I, Andersen P, Husar GM, Joos TO, Gennaro ML, Lochhead MJ, Denkinger CM, Perkins MD (2017) Diagnostic Performance of Tuberculosis-Specific IgG Antibody Profiles in Patients with Presumptive Tuberculosis from Two Continents. Clin Infect Dis 64: 947-955. PMI: 28362937

Manganelli R, Gennaro ML (2016) Protecting from Envelope Stress: Variations on the Phage-Shock-Protein Theme. Trends Microbiol. PMI: 27865622

Dutta NK, Bruiners N, Pinn ML, Zimmerman MD, Prideaux B, Dartois V, Gennaro ML, Karakousis PC (2016) Statin adjunctive therapy shortens the duration of TB treatment in mice. J Antimicrob Chemother 71: 1570-1577. PMI: 26903278

Chauhan R, Ravi J, Datta P, Chen T, Schnappinger D, Bassler KE, Balazsi G, Gennaro ML (2016) Reconstruction and topological characterization of the sigma factor regulatory network of Mycobacterium tuberculosis. Nat Commun 7: 11062. PMI: 27029515

Ascensao JA, Datta P, Hancioglu B, Sontag E, Gennaro ML, Igoshin OA (2016) Non-monotonic Response to Monotonic Stimulus: Regulation of Glyoxylate Shunt Gene-Expression Dynamics in Mycobacterium tuberculosis. PLoS Comput Biol 12: e1004741. PMI: 26900694

Vir P, Arrigucci R, Lakehal K, Davidow AL, Pine R, Tyagi S, Bushkin Y, Lardizabal A, Gennaro ML (2015) Single-Cell Cytokine Gene Expression in Peripheral Blood Cells Correlates with Latent Tuberculosis Status. PLoS One 10: e0144904. PMI: 26658491

Shi L, Salamon H, Eugenin EA, Pine R, Cooper A, Gennaro ML (2015) Infection with Mycobacterium tuberculosis induces the Warburg effect in mouse lungs. Sci Rep 5: 18176. PMI: 26658723

Salamon H, Bruiners N, Lakehal K, Shi L, Ravi J, Yamaguchi KD, Pine R, Gennaro ML (2014) Cutting edge: Vitamin D regulates lipid metabolism in Mycobacterium tuberculosis infection. J Immunol 193: 30-34. PMI: 24899504

Kunnath-Velayudhan S, Salamon H, Wang HY, Davidow AL, Molina DM, Huynh VT, Cirillo DM, Michel G, Talbot EA, Perkins MD, Felgner PL, Liang X, Gennaro ML (2010) Dynamic antibody responses to the Mycobacterium tuberculosis proteome. Proc Natl Acad Sci U S A 107: 14703-14708. PMI: 20668240

Shi L, Sohaskey CD, Kana BD, Dawes S, North RJ, Mizrahi V, Gennaro ML (2005) Changes in energy metabolism of Mycobacterium tuberculosis in mouse lung and under in vitro conditions affecting aerobic respiration. Proc Natl Acad Sci U S A 102: 15629-15634. PMI: 16227431

Davidow A, Kanaujia GV, Shi L, Kaviar J, Guo X, Sung N, Kaplan G, Menzies D, Gennaro ML (2005) Antibody profiles characteristic of Mycobacterium tuberculosis infection state. Infect Immun 73: 6846-6851. PMI: 16177363

Shi L, Jung YJ, Tyagi S, Gennaro ML, North RJ (2003) Expression of Th1-mediated immunity in mouse lungs induces a Mycobacterium tuberculosis transcription pattern characteristic of nonreplicating persistence. Proc Natl Acad Sci U S A 100: 241-246. PMI: 12506197

Goulding CW, Parseghian A, Sawaya MR, Cascio D, Apostol MI, Gennaro ML, Eisenberg D (2002) Crystal structure of a major secreted protein of Mycobacterium tuberculosis-MPT63 at 1.5-A resolution. Protein Sci 11: 2887-2893. PMI: 12441386

Colangeli R, Spencer JS, Bifani P, Williams A, Lyashchenko K, Keen MA, Hill PJ, Belisle J, Gennaro ML (2000) MTSA-10, the product of the Rv3874 gene of Mycobacterium tuberculosis, elicits tuberculosis-specific, delayed-type hypersensitivity in guinea pigs. Infect Immun 68: 990-993. PMI: 10639479