Arkady Mustaev, Ph.D.

Associate Professor of Microbiology, Biochemistry & Molecular Genetics
room W410-W

The research activity in Dr. Mustaev’s laboratory is focused on the development of chemical and physico-chemical approaches for studies of proteins, nucleic acids and their complexes. The object of long-term studies in the laboratory is bacterial RNA polymerase (RNAP). The main goal is understanding of the catalytic mechanism and regulation of RNA synthesis by RNAP at a molecular level of resolution.

New projects in the laboratory include: 1) drug discovery, aimed at finding a new inhibitors of bacterial transcription as potential drugs against microbial pathogens using high throughput screening (HTS). Resent activity in this direction was focused of development of fluorescence-based HTS-compatible assay for E.coli RNAP and adaptation of this assay for the enzyme from M. tuberculosis in collaboration with Dr. R. Ebright (Rutgers University). 2) Investigation of the mechanism of antibacterial and anti-cancer activity of isothiocyanates (ITC) – defense compounds of plants. This included the synthesis of highly active ITC derivatives and elucidation of their cellular targets. Collaborators: Drs. N. Kurepina and B. Kreiswirth (PHRI Center, Rutgers). 3) Studies of the inhibitory mechanism of fluoroquinolones on bacterial topoisomerases in collaboration with Dr. K. Drlica (PHRI Center, Rutgers). 4) Development of highly sensitive luminescent compounds as reporter groups for biological studies in collaboration with Drs. S. Marras, S. Tyagi (PHRI Center, Rutgers), and L. Krasnoperov (NJIT). 5) Development of new probes for tomographic diagnostics of fungal and yeast infections in collaboration with Dr. D. Perlin (PHRI Center, Rutgers).

View all on PubMed

Yurieva O, Nikiforov V, Jr., Nikiforov V, O’Donnell M, Mustaev A (2017) Insights into RNA polymerase catalysis and adaptive evolution gained from mutational analysis of a locus conferring rifampicin resistance. Nucleic Acids Res 45: 11327-11340. PMI: 29036608

Wiedman GR, Zhao Y, Mustaev A, Ping J, Vishnubhotla R, Johnson ATC, Perlin DS (2017) An Aptamer-Based Biosensor for the Azole Class of Antifungal Drugs. mSphere 2. PMI: 28861519

Mustaev A, Roberts J, Gottesman M (2017) Transcription elongation. Transcription 8: 150-161. PMI: 28301288

Lee MH, Wiedman G, Park S, Mustaev A, Zhao Y, Perlin DS (2017) A novel, tomographic imaging probe for rapid diagnosis of fungal keratitis. Med Mycol. PMI: 29228372

Kang JY, Olinares PD, Chen J, Campbell EA, Mustaev A, Chait BT, Gottesman ME, Darst SA (2017) Structural basis of transcription arrest by coliphage HK022 Nun in an Escherichia coli RNA polymerase elongation complex. Elife 6. PMI: 28318486

Mustaev A, Vitiello CL, Gottesman ME (2016) Probing the structure of Nun transcription arrest factor bound to RNA polymerase. Proc Natl Acad Sci U S A 113: 8693-8698. PMI: 27436904

Malik M, Mustaev A, Schwanz HA, Luan G, Shah N, Oppegard LM, de Souza EC, Hiasa H, Zhao X, Kerns RJ, Drlica K (2016) Suppression of gyrase-mediated resistance by C7 aryl fluoroquinolones. Nucleic Acids Res 44: 3304-3316. PMI: 26984528

Bick MJ, Malik S, Mustaev A, Darst SA (2015) TFIIB is only approximately 9 A away from the 5′-end of a trimeric RNA primer in a functional RNA polymerase II preinitiation complex. PLoS One 10: e0119007. PMI: 25774659

Bae B, Nayak D, Ray A, Mustaev A, Landick R, Darst SA (2015) CBR antimicrobials inhibit RNA polymerase via at least two bridge-helix cap-mediated effects on nucleotide addition. Proc Natl Acad Sci U S A 112: E4178-4187. PMI: 26195788

Mustaev A, Malik M, Zhao X, Kurepina N, Luan G, Oppegard LM, Hiasa H, Marks KR, Kerns RJ, Berger JM, Drlica K (2014) Fluoroquinolone-gyrase-DNA complexes: two modes of drug binding. J Biol Chem 289: 12300-12312. PMI: 24497635

Mekler V, Minakhin L, Borukhov S, Mustaev A, Severinov K (2014) Coupling of downstream RNA polymerase-promoter interactions with formation of catalytically competent transcription initiation complex. J Mol Biol. PMI: 25311862

Drlica K, Mustaev A, Towle TR, Luan G, Kerns RJ, Berger JM (2014) Bypassing Fluoroquinolone Resistance with Quinazolinediones: Studies of Drug-Gyrase-DNA Complexes Having Implications for Drug Design. ACS Chem Biol. PMI: 25310082

Wirpsza L, Krasnoperov L, Mustaev A (2013) New quinolone-based thiol-reactive lanthanide luminescent probes. J Photochem Photobiol A Chem 251: 30-37. PMI: 23833545

Sosunova E, Sosunov V, Epshtein V, Nikiforov V, Mustaev A (2013) Control of transcriptional fidelity by active center tuning as derived from RNA polymerase endonuclease reaction. J Biol Chem 288: 6688-6703. PMI: 23283976

Pratt A, Garcia-Effron G, Zhao Y, Park S, Mustaev A, Pillai S, Perlin DS (2013) Evaluation of fungal-specific fluorescent labeled echinocandin probes as diagnostic adjuncts. Med Mycol 51: 103-107. PMI: 22587729

Pillai S, Krasnoperov L, Mustaev A (2013) Simple no-chromatography procedure for amine-reactive Eu luminescent chelates optimal for bioconjugation. J Photochem Photobiol A Chem 255: 16-23. PMI: 23833546

Kurepina N, Kreiswirth BN, Mustaev A (2013) Growth-inhibitory activity of natural and synthetic isothiocyanates against representative human microbial pathogens. J Appl Microbiol. PMI: 23789822

Kozlov M, Nudler E, Nikiforov V, Mustaev A (2013) Reactive rifampicin derivative able to damage transcription complex. Bioconjug Chem 24: 443-447. PMI: 23425196

Wirpsza L, Pillai S, Batish M, Marras S, Krasnoperov L, Mustaev A (2012) Highly bright avidin-based affinity probes carrying multiple lanthanide chelates. J Photochem Photobiol B 116: 22-29. PMI: 23018156

Pilllai S, Kozlov M, Marras SAE, Krasnoperov LN, Mustaev A (2012) The synthesis and characterization of new cross-linkable quinolone and quinoline-based luminescent lanthanide chelates. Progresses in Nanotechnology and Nanomaterials 1: 18-24. PMI:

Pillai S, Kozlov M, Marras SA, Krasnoperov LN, Mustaev A (2012) New cross-linking quinoline and quinolone derivatives for sensitive fluorescent labeling. J Fluoresc 22: 1021-1032. PMI: 22450725

Marks KR, Malik M, Mustaev A, Hiasa H, Drlica K, Kerns RJ (2011) Synthesis and evaluation of 1-cyclopropyl-2-thioalkyl-8-methoxy fluoroquinolones. Bioorg Med Chem Lett 21: 4585-4588. PMI: 21705218

Malik M, Marks KR, Mustaev A, Zhao X, Chavda K, Kerns RJ, Drlica K (2011) Fluoroquinolone and quinazolinedione activities against wild-type and gyrase mutant strains of Mycobacterium smegmatis. Antimicrob Agents Chemother 55: 2335-2343. PMI: 21383100

Krasnoperov LN, Marras SA, Kozlov M, Wirpsza L, Mustaev A (2010) Luminescent probes for ultrasensitive detection of nucleic acids. Bioconjug Chem 21: 319-327. PMI: 20085336